Wednesday , 27 October 2021

Development and Validation of a RP-HPLC Method for the Determination of Acyclovir in Transdermal Patches

R. Venu Priya1 and Vinesh Kumar2
1Research Scholar, Sun Rise University, Alwar, Rajasthan, India.

2Department of Pharmacy, Sun Rise University, Alwar, Rajasthan, India.

The present report describes a rapid and sensitive High Pressure Liquid Chromatography (HPLC) method with UV detection to quantify acyclovir in transdermal patches. After sample preparation with diluent separation of acyclovir was achieved on a Kromasil C18 column (250mm×4.6mm, 5μm particle size) column at 30˚C. The mobile phase consisted of Buffer: Acetonitrile taken in the ratio 60:40 v/v, at a flow rate of 1.0 ml/min, with total run time of 8 min. Detection of acyclovir was done at 253 nm. Method was found to be selective, linear (r2 = 0.999), accurate (recovery, 100.22–100.31%) and precise (RSD, ≤0.52%) in the selected concentration range of 12.5-87.5ppm. Detection and quantitation limit of acyclovir in patch were 0.29 and 1.39ppm, respectively. Transdermal drug delivery systems are becoming more popular in the field of modern pharmaceutics. Different matrix-type transdermal films containing acyclovir was formulated with an objective to study the effect of polymers on the release characters. All the prepared formulations showed good physical stability. The in-vitro permeation studies were performed using Franz diffusion cell. Two in house formulations were selected as best formulations and further assayed by RP-HPLC method.
Keywords: Acyclovir, Transdermal patches, RP-HPLC, Method development, Method validation.

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