K.Krishna Chaitanya*, D. Gowri Sankar, Samson Israel. D, CH. Narasimha Kumar, BalaKumar.K,
CH.Harsha vardhan reddy.
Pharmaceutical Analysis & Quality Assurance Division
A.U. College of Pharmaceutical Science, Andhra University, Visakhapatnam-530003
A simple isocratic HPLC method has been developed and subsequently validated for determination of Cilostazol in pharmaceutical dosage forms. The method employs an Agilent Zorbax Eclipse XDB C18column (250mm x 4.60mm id, 5 μm Particle size) with flow rate of 1.0ml/min using UV detection at wave length 257nm. The separation was carried out using a mobile phase consisting of a mixture of 500ml of water, 350ml of Acetonitrile and 150ml of methanol. The retention time for Cilostazol was found to be 7.03minutes. A linear response was observed over the concentration range of 25-150µg/mL for the assay of Cilostazol. The limit of detection and the limit of quantification for Cilostazol were found to be 0.584 ppm and 1.020 ppm respectively. The results of analysis were validated statically and by recovery studies. Hence the proposed method was found to be accurate, precise, reproducible and specific and can be used for analysis of the drug in tablet formulation.
Key words: Cilostazol, Isocratic HPLC method, UV detection, Validation