Thursday , 29 July 2021

Synthesis, Characterization and In-vitro evaluation of Antibacterial Profile of 1, 4-dihydropyridine derivatives

Asish Bhaumik1*, Prasenjit Das2, Shibu Das3, M. Swapna4, Mahendar Boddupally5
1,4Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, India.
2,3Department of Pharmaceutical Chemistry, Regional Institute of Pharmaceutical Science and Technology, Abhoynagar, Agartala-799005, India
5Department of Pharmacology, Pratishta Institute of Pharmaceutical Sciences, Durajpally, Chivemla, Nalgonda-508214, Telangana State, India.

The objective of the present work was the synthesis of 1-[5-acetyl-4 (4-substituted phenyl)-2, 6-dimethyl-1,4-dihydroxypyridine-3-yl]-ethan-1-one and evaluation of  in vitro antibacterial activity. Based on this a new series of compound had been planned to synthesize by reacting acetyl acetone with various aromatic aldehydes in the presence of ammonium acetate. The in-vitro antibacterial activity was carried out by Paper disc diffusion method and MIC was determined by Agar streak dilution method. The results displayed that all the synthesized compounds had a potential antibacterial activity against different gram positive and gram negative bacteria with an MIC range of 9-22 µg/ml (gram positive organisms) and with an MIC range of 10-26 µg/ml (gram negative organisms). The MIC of the synthesized compounds (B1, B2, B3, B4, B5 and B6) for different gram positive bacteria were found to be S. aureus (MIC: 9-18 µg /ml ), B.subtilis (MIC: 10-21 µg /ml), S. typhi (MIC: 11-22 µg /ml) and S. epidermidis ( MIC:12-21 µg /ml). The synthesized compounds (B1, B2, B3, B4, B5 and B6) were active against all the tested gram negative microorganisms with the range of MIC values for P. aeruginosa (MIC: 10-20 µg/ml), P. flurocense (MIC:12-25 µg/ml) and E. coli ( MIC: 11-26 µg/ml) and V. cholerae (MIC:12-23 µg/ml). 
Keywords: IR, NMR, Antibacterial activity, Paper disc diffusion, MIC

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