Wednesday , 23 August 2017

Identification and Isolation of Bacterial bioremediation & Biotransformation of Pentachlorophenol by Synergistic Approach

Kumar Sunil*1, Tandan Neeraj2 and Bharadwaj Uma1
1Department of Microbiology, Maharaj Vinayak Global University, Rajasthan, Jaipur, University, India
2Scientific and Applied Research Centre, Meerut, India

A significant number of organic compounds present in pulp-paper mill effluent have been classified as mutagenic and clastogenic thereby turning these effluents into ‘a Pandora’s. The collected effluent was found slightly alkaline (pH-8.2) in nature and dark brown in colour with high level of chlorophenols PCP (220 mg/l); dichlorophenol (18.65) and trichlorophenol (10.73). Other pollution parameters like COD (15766±260 mg/l), BOD (6033±155mg/l), color 18534 CU, nitrates (35.3±3.0 mg/l) and sulphate (405±10.2 mg/l) were also found in high level. In this study 31 bacteria were isolated through enrichment culture technique. Three strains PPS-S6, S7 and S8were screened on the basis of various enzymatic activity (peroxidises and laccases enzymes) and identified as Pseudochrobactrum sp, Providencia rettgeri and Klebsiella pneumoniae, respectively on the basis of their biochemical properties.  Further the efficacies of these cultures were evaluated for the optimization of important factors which influencing the removal of PCP. The optimum condition for removal of PCP were optimized and results showed that a Carbon source 1%, nitrogen source 0.3% with agitation speed 120 rpm, temperature 30°C and pH 7.0 bent the finest grouping for boost the removal of PCP up to 94 %.  The HPLC and GC-MS of treated samples showed massive consumption of compounds by constructed consortium compared to control indicated that constructed consortium utilized constituents of effluent rather than biotransformation.
Keywords: PCP, GC-MS

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