Gundala Praveena*1, P.V. Madhava Reddy2
Ratnam Institute of pharmacy, Pidathapolur (V&P), Muthukur (M), SPSR Nellore (Dist) 524346, Andhra Pradesh, India.
A B S T R A C T
Anxiety is an unpleasant emotional experience of daily living characterized by a sense of apprehension, uneasiness or impending distress. This feeling is usually associated with changes in the autonomic nervous system and behaviour and it affects one-eighth of the total population worldwide and has become a very important area of research interest in psychopharmacology during this decade. The methanolic extract of Tricholepis glaberrima was subjected for the acute toxicity study to determine the therapeutic dose using albino rats in controlled environment. Acute oral toxicity study was performed as per OECD-423 guidelines. Tricholepis glaberrima showed maximum analgesic activity at 60, 90 min for 100 and 200mg/kg dose. The reaction time in normal control group at 60, 90 min was found to be 0.77±0.22, 0.78±0.23 sec. The reaction time (paw licking / jumping response) in rats pretreated with lower dose of Tricholepis glaberrima (100mg/kg), higher dose of Tricholepis glaberrima (400mg/kg/day) and Diclofenac sodium (9 mg/kg) at 60, 90 min were found to be 2.82±0.52, 2.98±0.36, 4.02±1.02 and 4.58 ± 0.36 respectively when compared to control group rats. The duration of analgesic effect was more in 200 mg/kg compared to 100 mg/kg and reference drug diclofenac sodium at 9 mg/kg dose significantly increased the reaction time at 90 minutes.
Keywords: Analgesic activity, Methonolid extract, In-Vivo models and albino rats.