Analytical Method Development and Validation by RP-HPLC for Simultaneous Estimation of Lopinavir and Ritonavir in Combined Tablet Dosage form

Gampa Vijaya Kumar¹*, N. Rajendra Prasad^{2                                                                                                                           
1}Professor, Departmernt of Pharmaceutical Analysis, CVM College of Pharmacy, Velichala, Kothapally, Karimnagar, Telangana-505451
²Asst. Professor, Departmernt of Pharmaceutical Analysis, CVM College of Pharmacy, Velichala, Kothapally, Karimnagar, Telangana-505451

A B S T R A C T
A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Lopinavir and Ritonavir in pharmaceutical dosage form. The mobile phase consisted of Methanol : phosphate buffer (70:30) 0.8ml /10 min and wavelength of detection at 260 nm. The retention times of were Lopinavir and Ritonavir 3.527 min and 3.003 min respectively. Chromatograms was run through Inertsil ODS C₁₈ (4.6, 150mm, 5μm). The % RSD of the Lopinavir and Ritonavir were found to be 2%.  The % Recovery was obtained 98-102% of Lopinavir and Ritonavir. The linearity range of Lopinavir and Ritonavir 100-500μg/ml. The method was validated in terms of linearity, precision, accuracy, limit of detection, limit of quantification.. The coefficient of variance for both the drug was more than 0.999.The proposed method can be used for determination of these drugs in combined dosage forms.
Keywords: Lopinavir, Ritonavir, RP-HPLC