Thursday , 11 July 2024

Antitumor Activity (In-vitro) of Extracellular Polysaccharide Produced by Ropy Lactobacillus delbrueckii ssp. bulgaricus Isolated from Tradisional Fermented Milk

Ratmawati Malaka*, Efendi Abustam and Sudirman Baco
Hasanuddin University, Faculty of Animal Science, Jl. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar, Indonesia.

An extracellular polysaccharide (EPS) was purified by ethanol precipitation from culture supernatant of Ropy strains of Lactobacillus delbrueckii ssp. bulgaricus, a lactic acid bacteria that was isolated from commercial yoghurt.  The EPS was produced under aerobic conditions in a medium Skim Milk Reconstitution (SMR) 10% (w/v). This EPS have studied to potential health – promoting effect as antitumor activity.  The objective of this study was to determine of potential of the EPS on proliferation of tumor cell in vitro. EPS with different concentration (0.01; 0.02; 0.05; and 0.001 mg/ml) was examined in vitro for antitumor activity on Leukemia K-562 and Hela cell.  Result of these studies had showed that EPS in doses 50 mg/ml can inhibited of 45.4 % of tumor leukemia and 59.2% for tumor human cervix (Hela cell).
Keywords: antitumor activity, Lactobacillus bulgaricus, antiproliferation, exopolysaccharide (EPS).

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