SK. Shahina*, M. Gobinath, V. Haribaskar, Ramesh Dhani
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Muthukur (M), Nellore, A.P, India.
A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Fluoxetine Hcl and Olanzapine in Tablet dosage form. Chromatogram was run through Inertsil C18 ODS column (250mm 4.6mm, 5µ). Mobile phase containing Acetonitrile and methanol in the ratio of 90:10A was pumped through column at a flow rate of 1ml/min. Optimized wavelength for Fluoxetine Hcl and Olanzapine was 233nm. Retention time of Fluoxetine Hcl and Olanzapine were found to be 2.9 min and 3.5 min. %RSD of the Fluoxetine Hcl and Olanzapine were and found to be 0.12 and 0.36 respectively. %Recover was Obtained as 98.0% and 101.5% for Fluoxetine Hcl and Olanzapine respectively. LOD, LOQ values are obtained from regression equations of Fluoxetine Hcl and Olanzapine were 0.41ppm, 0.16ppm and 1.25ppm, 0.48ppm respectively. Regression equation of Fluoxetine Hcl is y = 18305.x + 366.7, and of Olanzapine is y = 5411x + 159.5
Keywords: Fluoxetine Hcl, Olanzapine, RP-HPLC.