A. Malli Babu1, Dr. P. Janaki Pathi*2, Dr. N. Appala Raju3
1Analytical Department, Hospira Healthcare India Pvt. Limited, Chennai, India
2Analytical Department, Vishnu Chemicals Limited, Hyderabad.
3Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Hyderabad-500 034
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Taineptine in tablet dosage form. An Inertsil ODS-3V analytical column (250 x 4.6 mm, 5 μm partical size) with mobile phase consisting of mixture of buffer 0.1% ortho-phosphoric acid in water and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 220 nm. The retention time was 8.6 min. The detector response was linear in the concentration of 1-12 mcg/mL. The respective linear regression equation being y=1025.6x-1028.4. The limit of detection and limit of quantification was 0.005mcg /mL and 0.015mcg/mL respectively. The percentage assay of Taineptine was 99.6 %. The method was validated by determining its accuracy, precision and linearity. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Taineptine in bulk drug and in its pharmaceutical tablet dosage form.
Keywords: Taineptine, RP-HPLC and Tablets