Sarkodie JA1*, Edoh DA4 , Kretchy IA2 , Aboagye FA4, AppiahAA4, Frimpong-Manso S1, Asiedu-Gyeke JI3 , Nyarko AK3, Debrah P1 , and Owusu Donkor P1
1Department of Pharmacognosy and Herbal Medicine, University of Ghana, Accra-Ghana
2Department of Pharmacy Practice and Clinical Pharmacy, University of Ghana, Accra-Ghana
3Department of Pharmacology and Toxicology, School of Pharmacy, University of Ghana, Accra-Ghana
4Department of Phytochemistry, Centre for Plant Medicine Research, Mampong-Akwapim, Ghana
Backgroud: Croton membranaceus is a shrub which grows in most African countries mainly the coastal belt. It is an important medicinal plant used by Ghanaian Traditional Medical Practitioners in the treatment of benign prostatic hyperplasia (BPH) and diabetes. The present study aims to investigate the effects of 96% ethanol and aqueous extracts of C. membranaceus. Methods: The powdered root (3 kg) was separately cold macerated using 96% ethanol and aqueous. The doses for the study were fixed based on the Irwin test. The hypoglycaemic effect of both extracts (with doses 100 mg/kg, 600 mg/kg) was studied in streptozotocin-induced diabetic rats. Results: The extracts caused reduction of glucose levels after 6 hours and 28 days of administration in the streptozotocin-induced diabetic rats. Compound N[N-(2-methylbutanoyl) glutaminoyl]-2-phenylethylamine was isolated from C. membranaceus and may be one of the active constituents responsible for the observed activity. Conclusion: The findings demonstrate the potential of both ethanolic and aqueous extract of C. membranaceus on type 1 diabetes mellitus, justifying the traditional use of the plant in the management of diabetes by traditional healers in Ghana.
Keywords: Croton membranaceus, N [N-(2-methylbutanoyl) glutaminoyl]-2 phenyl ethylamine, streptozotocin, diabetes