Farhana Shaheen*, Salma Begum, Rubina Kauser, Airaj Mahajabeen, MD Muzammil Hassan
MRM College of Pharmacy, Chintapalliguda, Ibrahimpatnam, R.R District-501510
A B S T R A C T
Reverse phase high-performance liquid chromatography (RP-HPLC) involves the separation of molecules on the basis of hydrophobicity. The separation depends on the hydrophobic binding of the solute molecule from the mobile phase to the immobilized hydrophobic ligands attached to the stationary phase, i.e., the sorbent. A schematic diagram showing the binding of a peptide or a protein to a reversed-phase surface. The literature review reveals the few UV and HPLC methods for the estimation of Olodaterol and tiotropium alone and in combination with other drugs. Few methods are also reported for estimation of both drugs from formulation .we intend to develop a RP-HPLC and UV methods by simultaneous determination with simple, rapid, greater sensitivity and faster elution.
Keywords: olodaterol, tiotropium, RP-HPLC