Farhana Shaheen*, Rubina Kauser, Salma Begum, Airaj Mahajabeen, Md Fasiuddin
MRM College of Pharmacy, Chintapalliguda, Ibrahimpatnam, R.R District-501510
A B S T R A C T
Introduction Reversed-phase high-performance liquid chromatography (RP-HPLC) involves the separation of molecules on the basis of hydrophobicity. The separation depends on the hydrophobic binding of the solute molecule from the mobile phase to the immobilized hydrophobic ligands attached to the stationary phase, i.e., the sorbent. A schematic diagram showing the binding of a peptide or a protein to a reversed-phase surface. The literature review reveals few HPLC methods for the estimation of Artemether and Lumefantrine alone and in combination with other drugs. Few methods are also reported for estimation of both drugs from formulation .we intend to develop a Stability indicating RP- HPLC method by simultaneous determination with simple, rapid, greater sensitivity and faster elution. The solute mixture is initially applied to the sorbent in the presence of aqueous buffers, and the solutes are eluted by the addition of organic solvent to the mobile phase. Elution can proceed either by isocratic conditions where the concentration of organic solvent is constant, or by gradient elution whereby the amount of organic solvent is increased over a period of time. The solutes are, therefore, eluted in order of increasing molecular hydrophobicity. RP-HPLC is a very powerful technique for the analysis of peptides and proteins.
Keywords: Artemether and Lumefantrine, RP-HPLC, method development