R. Sudheer kumar*, M. Gobinath, V. Haribaskar, Ramesh Dhani, V. Pavan kumar
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Nellore, Andhra Pradesh, India
A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Nifedipine and Triamterene in Tablet dosage form. Chromatogram was run through Inertsil-ODS C18, 250x 4.6 mm, 5m. Mobile phase containing Methanol and Water in the ratio of 80:20 was pumped through column at a flow rate of 1ml/min. Optimized wavelength for Nifedipine and Triamterene was 238nm. Retention time of Nifedipine & Triamterene were found to be 2.955min and 3.538 min. %RSD of the Nifedipine and Triamterene were and found to be 0.27 and 0.21 respectively. %assay was obtained as 99.24% and 99.82% for Nifedipine and Triamterene respectively. LOD, LOQ values are obtained from regression equations of Nifedipine & Triamterene were 0.08ppm, 0.25ppm and 0.03ppm, 0.08ppm respectively. Regression equation of Nifedipine is y= 10831x – 34273, and y = 21030x + 31232 of Triamterene.
Keywords: Nifedipine, Triamterene, RP-HPLC