Sk. Karimulla, Challa Sujitha*, M. Gobinath, B. Bharathi
Department of Pharmacology, Ratnam Institute of Pharmacy, Pidathapolur, Muthukur, SPSR Nellore, A.P, India.
A B S T R A C T
To evaluate the potential spermatotoxic effect of ethanolic extract of Polyalthia suberosa in male albino rats. Ethnolic extract of Polyalthia suberosa were given by gavage to rats in the in vivo test at a dose of 200 and 400mg/kg of bodyweight, along with normal controls and their by studying changes in sperm morphology consisting counts, motility and abnormalities of cauda epididymal sperm adapting light microscopy. Finding of this study revealed, the sperm concentration in the epididymis and sperm motility are decreased, whereas sperm abnormalities increased like sloughing of sperm neck, detached head, and coiling of end tail. In extract treated rats, the duration of sperm motility reduced with respect to the increased dose level. A highest 2.09-fold (p < 0.005) and 1.28-fold (p < 0.001) reduction in the sperm counts and the viability, respectively were observed in Polyalthia suberosa 400mg/kg body weight group. Sperm abnormality was increased by Polyalthia suberosa in a dose-dependent manner was assessed by acridine Orange fluorescent staining and there was a highest elevation by 2.2-fold (p < 0.001) in 400mg/kg group .This result indicates disruption of spermatogenic as well as androgenic compartment. The present study can be concluded the Polyalthia suberosa extracts suppress male fertility without altering the general metabolism.
Keywords: Polyalthia suberosa, spermatotoxic effect, wistar albino rats, ethanolic extract