Anurag Singh*1, Dr. LVG Nargund2
1Research scholar, CU Shah University, Wardhman City, Gujrat
2Director and Principal, Nargund College of Pharmacy, Bangalore, Karnataka
A B S T R A C T
Drug discovery, synthesis and development multidisciplinary, creative, complex and highly regulated process Heterocyclic compound are organic compound containing oxygen, sulphur and nitrogen. New drug synthesis not only done loping new chemical entities but also important for therapeutic need .In view of the general observation that pharmacological activity is invariably associated with a large variety of heterocyclic compounds, the present investigation of heterocycles belonging to class of cinnoline was undertaken. These compounds are reported to possess a wide spectrum of biological activity which includes antibacterial, antifungal, anticonvulsant, anticancer, anti-inflammatory, antihypertensive and analgesic activities. The current study involves the synthesis of 6-sulphonamido-cinnolines and their purification and characterized reaction .and were screened for antibacterial anti-oxidant and in vitro anti-inflammatory activity using bovine serum albumin denaturation model Sulphanilamide was treated with sodium nitrite in presence of conc. HCl at 0-5 °C to form diazonium salt, which on treatment with cyanoacetamide gave aryl hydrazine (cyano) acetamide. It was allowed to react with anhydrous AlCl3 in the presence of chlorobenzene to form 4-amino-6-sulphonamido-3-cinnolino carboxamides. This on treatment with form amide gave sulphonamido-cinnolino-pyramidine.6-sulphonamide cinnoline derivative identified and characterized by physical methods like m.p, TLC, and spectral method UV, IR, and NMR data. The newly synthesized compounds were investigated for their microbial susceptibility using in vitro model for against bacillus subtillis (gram +ve) Escherichia coli (gram – ve) the compounds were also subjected for bovine serum albumin denaturation which was considered as in vitro anti-inflammatory model. The newly synthesized compounds 4, 5,9, 15 and 17 exhibited good antibacterial activity. Other compounds exhibited weak activity. Compounds 5, 6, 7 and 13 showed good anti-inflammatory activity. Other synthesized compounds (4, 12 and16) exhibited weak activity.
Keywords: Sulphanilamide, anti-bacterial, anti-inflammatory, anti-oxidant