Monday , 24 June 2024

Simultaneous Estimation of UV Spectroscopy and Method Development and Validation for Lansoprazole and Domperidone by RP-HPLC

Sanju Srinivas1, Marium Nazneen2
1Associate Professor Department of Pharmacy, Ganga Pharmacy College, Nizamabad, Telangana
2Department of Pharmaceutical Analysis, Ganga Pharmacy College, Nizamabad, Telangana

A new method was established for simultaneous estimation of Lansoprazole and Domperidone by RP-HPLC method. The chromatographic conditions were  successfully developed  for  the  separation  of  Lansoprazole and Domperidone by using Xterra C18 5µ m (4.6*250mm) column, flow rate was 1ml/min, mobile phase ratio was Phosphate buffer (0.05M) pH 4.6: ACN (55:45%v/v)   (pH was   adjusted   with   orthophosphoric acid), detection wave length was 255nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, PDA Detector 996, Empower-software version-2. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)).  The  linearity study for Lansoprazole, Domperidone was found in concentration range of 1μg-5μg and 100μg-500μg and correlation coefficient (r2) was found to be 0.999 and 0.999, % mean recovery was found to be 100% and 100.5%, % RSD for repeatability was 0.7 and 0.4,% RSD for intermediate precision was 0.18 and 0.39 respectively.
Keywords: Lansoprazole, Domperidone, RP-HPLC, validation.

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