Monday , 10 May 2021

In-Vitro Antibacterial Activity of Harungana madagascariensis Lam. Ex. Poir. (Hypericaceae) stem bark extracts on some enterobacteria producing extended spectrum β-lactamases (ESBLS)

Abalé Anatole Toty1,2*, Nathalie Kouadio Guessennd2, Albert Dama Otokore1, Moreno Galleni3, Jules N’Guessan Kouadio4, Joseph Allico Djaman1, Mireille Dosso2
1Laboratoire de Pharmacodynamie Biochimique, UFR Biosciences, Université Félix Houphouët Boigny d’Abidjan, 22 BP 582 Abidjan 22 Côte d’Ivoire.
2Laboratoire de Bactériologie-Virologie, Unité des Antibiotiques, des Substances Naturelles et de la Surveillance des Résistances des Microorganismes aux Anti-Infectieux (Assurmi) de l’Institut Pasteur de Côte d’Ivoire, 08BP1563 Abidjan 08.
3Centre d’Ingénierie des Protéines, Université de Liège, Institut de Chimie B6a, Allée du 6 Août, 11 Start Tilman-B4000, Liège, Belgique.
4UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801 Abidjan 02.

H. madagascariensis is well known for its antibacterial properties against various bacterial genera. The objective of this study was to evaluate the antibacterial activity of bark extracts against enterobacteria producing extended spectrum β-lactamase (ESBLs). Methods agar diffusion and dilution in liquid medium were used. Only the hydro-ethanol extract was more effective inhibition with diameters ranging from 11 mm to 15 mm. The phytochemical analysis of this extract showed the presence of polyphenols, flavonoids, tannins and saponins. Antibacterial parameters showed that the extract was bactericidal on 66.67% of strains and bacteriostatic on 33.33% strains. The minimum inhibitory concentrations (MIC) were ranging from 1.56 mg/ml to 100 mg/ml while minimum bactericidal concentrations (MBC) were ranging from 50 mg/ml to 100 mg/ml. This study shows that H. madagascariensis could be a good candidate in the search for new active compounds based on herbal against enterobacteria producting β-lactamase extended spectrum.
Keywords: H. madagascariensis, enterobacteria, β-lactamase extended spectrum.

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