K. Narsu kumari*, Padma R1, Ch. Bala Sudhakar2, A. Sahithi Sri3, Jyothi P4, Veena Bandaru5
*12345A.M. Reddy Memorial College of Pharmacy, Narasaraopet, 522412, A.P, India
A B S T R A C T
In the method development for RP-HPLC method for Ciprofloxacin and Dexamethasone in bulk drug dosage form with Acetonitrile and Buffer (Phosphate buffer pH: 3) in the ratio 60:40 used as Diluents 8 mints run time. Method was optimized by varying chromatographic parameters like column, mobile phase composition, mobile phase PH and flow rate to satisfy system suitability testing. Various columns and mobile phase combinations were tried. A satisfactory separation and good peak symmetry was obtained by using Agilent C18 (4.6x250mm) column, 10mM Phosphate buffer (pH 3.0): Acetonitrile (20:80) as mobile phase with Isocratic technique. Quantification was achieved with UV detection at235 nm based on peak area. The assay results obtained by using the proposed method for the analysis of marketed ophthalmic solution containing Ciprofloxacin 5mg and Dexamethasone 50mg were in good agreement with the labeled amounts of Ciprofloxacin and Dexamethasone. The average contents of Ciprofloxacin and Dexamethasone were10mg/ml (99.7 %) and100mg/ml (99.8%) respectively.
Keywords: Ciprofloxacin, Dexamethasone, Accuracy, Precision, Acceptance criteria