M. Razia1* and Sivaramakrishnan2
1Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal, India
2Department of Biotechnology, Bharathidasan University, Tiruchirappalli, India
Chloroform extract of leaves and flowers of Datura metel was assessed for phytochemical components, antioxidant and antimicrobial activity. The preliminary phytochemical investigation was done for both leaves and flowers revealed that contained alkaloids, terpenoids, phenolic compound, tannins, saponin and glycoside. In addition to this, inhibitory effects on six pathogenic organisms of Klebsiella pneumoniae, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa, Proteus mirabilis, and Escherichia coli were performed. The results showed that leaves extract exhibited the highest inhibitory activity against S. typhi (25mm), followed by S. flexneri (21mm) and the least activity against K. pneumoniae (16mm), E. coli (15mm) at the concentration of 100µg whereas flowers extract showed S. typhi (20mm), followed by S. flexneri (19 mm) and the least activity against K. pneumoniae (13mm), E. coli (9mm). The leaves extract revealed the highest reducing power and H2O2 activity than flowers.
Keywords: Datura metel, Phytochemicals, Antibacterial activity, Antioxidant activity