Amit Gupta and Sushama R. Chaphalkar*
Department of Immunology, Vidya Pratishthan’s School of Biotechnology, Vidyanagari, Baramati-413133, Pune, India
A B S T R A C T
Anti-inflammatory activity of aqueous extract of leaves of Prosopis spicigera (50, 100 and 300 mg/kg body weight) in Swiss mice using cyclophosphamide as an immunosuppressant and was evaluated the antibody (IgG) titre, peritoneal macrophages activation and estimates the T cell surface markers i.e. CD3, CD4 and CD8 and also analyzed the Th1 type of cytokines (IFN-gamma and TNF alpha) in serum. Mice were immunized with cyclophosphamide two days prior to immunization with antigen bovine serum albumin (BSA, 1 mg/ml). The results showed that the aqueous extract of Prosopis spicigera showed a significant decrease in the antibody (IgG) titre, peritoneal macrophage activation and T cell surface markers i.e. CD3, CD4 and CD8 at a dose range of 300 mg/kg as compared to control. No mortality was occurred in all the tested drug samples. Overall, the aqueous extract showed anti-inflammatory effect on the cell mediated immune (T cell surface markers) response, macrophage activation and Th1 type of immune cytokines functions in mice.
Keywords: Prosopis spicigera, bovine serum albumin, cyclophosphamide