Monday , 24 June 2024

Analytical Method Development and Validation for the Simultaneous Estimation of Celecoxib and Diacerein in Bulk and Pharmaceutical Dosage Form Using RP-HPLC

Ameena Parveen1*, Dr. R. Vani2, Dr. M. Sunitha
1Post-graduate student, Shadan Women’s College of Pharmacy, Hyderabad, India

2Head of the department, Pharmaceutical Analysis, Shadan Women’s College of Pharmacy, Hyderabad
3Principal, Shadan Women’s College of Pharmacy, Hyderabad, India

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Celecoxib and Diacerein in bulk and pharmaceutical dosage form using C18 column (Waters, 250 x 4.6 mm, 5μm) in isocratic mode. The mobile phase consisted of a mixture Phosphate buffer pH (3.5), and Acetonitrile (HPLC grade) in the ratio of 20:80 v/v%. The detection was carried out at 261 nm for both Celecoxib and Diacerein. The method was linear over the concentration range of60-140μg/ml for Celecoxib and 30-70μg/ml for Diacerein. The recoveries of Celecoxib and Diacerein were found to be 99.38% and 100.61% respectively. The validation of method was carried out According to ICH guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

Keywords: Celecoxib, Diacerein, RP – HPLC, validation

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