Sunday , 3 March 2024

RP-HPLC Method for the Simultaneous Estimation of Ezetimibe and Atorvostatin in Bulk and Tablet Dosage Form

M. Madhuri1, P. Sowjanya2, Dr. Gampa Vijaya Kumar*3
1,2KGR Institute of Technology and Management,   Rampally,   Kesara,   Rangareddy, Telangana, India,

3Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India

A new method was established for simultaneous estimation of Ezetimibe and Atorvastatin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Ezetimibe and Atorvastatin by using ThermosilC18 column (4.0×125mm) 5µ, flow rate was 0.7ml/min, mobile phase ratio was (70:30 v/v) methanol: Sodium acetate buffer pH was adjusted to 3 with orthophosphoricacid, as to maintain biorelevant media specifications detection wavelength was 252nm. The instrument used was Shimadzu LC-20AD, SPD-M20Adiodearraydetector and LC20 solutions software. The retention times were found to be 2.566 mins and 3.417 mins. The % purity of Ezetimibe and atorvastatin was found to be 100.7% and 101.4% respectively. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1))

Keywords: Thermosil C18 column, Ezetimibe and Atorvastatin, RP-HPLC

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