Thursday , 15 November 2018

Simultaneous Estimation of Amlodipine Besylate and Perindopril Erbuminein combined Dosage form by RP-HPLC

1Tribhuvan Singh*,2M. Purushothaman,3Jogu Chandrudu, 4S. Mohammed Yusuf
1Scient Institute of Pharmacy, Ibrahimpatnam, Hyderabad, India

A B S T R A C T
The present work was developed and validated an analytical method for quantitative determination of Perindopril Erbumine and Amlodipine Besylate in a tablet formulation. Chromatographic separation of the two drugs was achieved on an Eclipse XDB C-8 (150 mm X 4.6 mm), 5mm. The mobile phase constituted of Buffer: Acetonitrile (65:35) and pH adjusted to 2.6 with dilute Ortho- Phosphoric Acid was delivered at the flow rate 1.0 mL/min. Detection was performed at 210 nm. Separation was completed within 8 min. The retention time for Perindopril Erbumine and Amlodipine Besylate 3.168 & 5.504 min respectively. Calibration curves were linear with correlation coefficient between 0.99 to 1.0 over a concentration range of 8 to 60 mg/mL of Perindopril Erbumine and 10 to 75 mg/mL of Amlodipine Besylate. The relative standard deviation (RSD) was found< 2.0%.
Keywords: Perindopril Erbumine; Amlodipine Besylate; Reversed-phase HPLC.

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