Thanuja*, Sk. Phareeda, Prasanthi.A, K. Srilatha
Department of Pharmacology, Ratnam Institution of Pharmacy, Pidathapolur, Muthukur, Nellore, Andhra Pradesh
A B S T R A C T
Objectives: The objective of this study is to evaluate the neuroprotective effect Global cerebral ischemia was induced by temporary bilateral carotid artery occlusion followed by reperfusion (Oxidative stress). Materials and Methods: Albino wister rats of wither gender were used in the study. Evaluation of neuroprotective activity of flavonoid Clerodandrum serratum (in200 and 400 mg/kg oral doses) was carried out by using the global cerebral I/R model by bilateral carotid artery occlusion for 10 min, followed by 24 h reperfusion. The antioxidant enzymatic and non-enzymatic levels were estimated along with histopathological studies. Result: Clerodandrum serratum showed dose-dependent neuroprotective activity by significant decrease in lipid peroxidation (P < 0.001) and increase in the superoxide dismutase, catalase, glutathione and total thiol levels in gossypin treated groups when compared to control group. Cerebral infarction area was markedly reduced in Clerodandrum serratum treated groups when compared to control group. Conclusion: Clerodandrum serratum showed potent neuroprotective activity against global cerebral I/R injury- induced by temporary bilateral carotid artery occlusion followed by reperfusion (oxidative stress) in rats.
Keywords: Neuroprotective, flavonoids, Clerodandrum serratum, ischemia/reperfusion, natural products, oxidative stress.