P. Jaya Preethi*
Department of Pharmacy, Nandha College of Pharmacy and Research Institute,
Koorapalayam Piruvu, Erode, TamilNadu, India.
The present paper deals with isolation, characterization and evaluation of Anti-diabetic and CNS Stimulant activities of methanolic extract of Scutia myrtina. Extraction of whole plant was carried out with soxhlet apparatus using solvents- petroleum ether and methanol. The extract was subjected to Preliminary Phytochemical Screening for the presence of different chemical groups. The plant contains phtochemicals such as alkaloids, glycosides, tannins, flavonoids, triterpenoids, fixed oils and fats. Flavonoids are isolated using the solvent system toluene:ethylacetate (5:1). The methanolic extract of whole plant of Scutia myrtina at the dose of 400 mg/kg was administered orally once a day to the groups for 21 days. The plant extract significantly (p<0.001) decreased the levels of Glucose, Cholesterol, Triglycerides, SGOT and SGPT and also significantly (p<0.001) increased the level of Total protein. The methanolic extract produced significantly (p<0.001) 20.11% increase in locomotor activity when compared to the Normal Control. The methanolic extract had no significant effect on rota rod. The extract significantly (p<0.05 & p0.001) decreased the no. of entries and the time spent in the open arm in the elevated plus maze. The extract also significantly (p<0.001) increased the no of entries in the Y maze. It also significantly (p<0.001) decreased the immobility time and increased the frequency of swimming and climbing in forced swim test. The study concluded that methanolic extract of whole plant of Scutia myrtina at the dose of 400 mg/kg can be used as anti-diabetic and CNS stimulant.
Key words: Anti-diabetic, CNS Stimulant, Isolation, Phytochemical analysis, Scutia myrtina