G. Sravanthi*1, Dr. G. Nagarjuna Reddy2, Rajani Gunnam3, Dr. J.V. Suresh4, G. Harika 5
1Research Scholar, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
2Professor and Principal, Department of Pharmaceutical Chemistry, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
3Assistant Professor, Department of Pharmacy Practice, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
4Associate Professor, Department of Pharmacology, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
5Department of Pharmacology, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
A B S T R A C T
To investigate the Nephroprotective effect of ethanolic extract of leaf Artemesia vulgaris (EEAV) against diabetes induced nephropathy in rats. Diabetes was induced to rats by intraperitoneal injection of streptozotocin at a dose of 60mg/kg. Past 48 hours of STZ administration, blood samples were collected and the induction of diabetes mellitus was confirmed by plasma glucose level determination. The rats with plasma glucose level 250 mg/dl are randomly divided into five groups and each group consists of 6 animals. EEAV of doses 250 & 500 mg/kg b.w was administered to streptozotocin induced diabetic nephropathy rats orally continuously for a phase of 16 weeks. Another group of diabetic rats were treated with aminoguanidine hydrogen carbonate (AMG) in drinking water at the dose of 1mg/ml continuously for a period of 16 weeks. Whereas, diabetic nephropathy control rats treated with vehicle. At the end of 16 weeks of protocol, animals were sacrificed; blood was collected, processed for collection of serum and stored at -70◦C until further use. The serum is utilized for estimation of various diagnostic markers like plasma glucose, plasma creatinine etc. by utilizing standard kits to confirm the chronic glycemia, and to assess the kidney function. Kidneys are collected immediately after sacrification, kept in formalin solution and processed for histopatholgical observation for favourable effect of EEAV. Animals treated with EEAV for a period of 16 weeks exhibited the significant decrease in diabetic evaluation parameters like blood glucose when compared to diabetic nephropathy rats. Daily treatment with two dose levels of EEAV up to 16 weeks, exhibited the significant dose dependant decrease in different renal function parameters like urinary albumin excretion rate (UAER), type IV collagen Excretion, total proteins in urine, creatinine, advanced glycation end products, excretion of glycosaminoglycans and significant increase in glycosaminoglycans, urine volume, urinary creatinine and GFR when compared to diabetic nephropathy rats. Histological observation of the kidney of diabetic nephropathy rats exhibited the alteration of normal morphological changes. Kidneys isolated from animals treated with both concentrations of EEAV shows recoverable morphological changes. The results of our study reveal that EEAV reduces the renal impairment in diabetic nephropathy rats through anti-diabetic, antioxidant and protective morphological changes.
Keywords: Artemesia vulgaris, Antihyperglycemic, Anti- Oxidant, Diabetic Nephropathy.