K. Saravana Kumar*, Nagaveni P, Prasanna Raju Y, Mahaboob Subahan T
Department of Pharmaceutics,
Sree Vidyanikethan College of Pharmacy,Tirupati, Andhra Pradesh, India
The objective of the present research work was to evaluate the effect of antiarthritic activity of ethanolic extract of Vitex Negundo in laboratory animals.
For arthritic study, the animals were divided randomly into 5 groups with 3 rats per group as following: Group I- vehicle control, Group II Arthritic control, Group III VNE (100mg/kg.p.o), Group IV VNE (200 mg/kg.p.o), Group V standard i.e. Indomethacin (10 mg/kg, p.o.). On day 1 and day 3, 0.1 ml of 2 % v/v formaldehyde in normal saline was injected into the subplantar region of the left hind paw.
Dosing with standard drug (Indomethacin) and extracts were started on same day and continued for 10 days. The serum separated from the blood was collected for further biochemical estimations. Arthritis was assessed by following parameters as body weight, arthritic score, non injected, injected paw volume, biochemical estimations and haematological studies like Hemoglobin content, red blood cell, white blood cell counts and erythrocyte sedimentation rate. A significant increase in body weight, reduction in paw volume of both hind legs and reduction in total arthritic score were observed in formaldehyde induced arthritis in rats. There was a significant improvement in the levels of Hb and RBC in Vitex Negundo treated rats. The increased levels of WBC, ESR were significantly suppressed in the extract administered arthritic group.
In the present study, we have evaluated Anti-Arthritic effects induced by formaldehyde, ethanolic extract of Vitex Negundo. Linn. This significantly suppressed the swelling of the paws of rats as compared to standard Indomethacin on dose dependent manner.
Key words: Antiarthritic activity, biochemical estimations, Vitex Negundo, and RA test.