C. Karuppasamy*1, Sanjay Kumar Jain2
1Safa College of Pharmacy, Kurnool, Andhra Pradesh, India
2Department of Pharmaceutical Analysis, Bundelkhand University, Jhansi, U.P, India
A B S T R A C T
The aim of present research work simple, precise, accurate and robust RP-HPLC method for the simultaneous estimation of Clopidogrel and Atorvastatin in bulk and tablet dosage form. The chromatographic separation was performed by Waters C 18 (4.6 x 250mm, 5.0mm) column and the mobile phase was consisting of OPA buffer: Methanol in the ratio of 25:75. The flow rate was maintained at 1.0 ml/min and UV detection of wavelength was observed at 227 nm. The linearity was found to be 1µg/ml to 5µg/ml of Clopidogrel, 7.5µg/ml to 37.5µg/ml of Atorvastatin with correlation coefficient was found to be 0.999. The percentage RSD was found to be less than 2. All the validation parameters are stastically validated with according to ICH guidelines. So, the developed method was can be applied to the quality control analysis of analytical laboratories for the simultaneous estimation of clopidogrel and Atorvastatin.
Keywords: Clopidogrel, Atorvastatin, RP-HPLC, Mobile phase, Validation