Prem Sundar Pokuru*, P. Sailaja, C. Madhavi Latha, Y. Prapurna Chandra
Department of Pharmacology, Ratnam Institute of Pharmacy, Pidathapolur (V&P), Muthukur (M), SPSR Nellore– 524 346
A b s t r a c t
Oxidative stress has been implicated in the connection of diseases such as diabetes, cardiovascular diseases, liver cirrhosis nephrotoxicity, cancer, and aging, etc. Antioxidant substrates from plants may offer resistance against the oxidative stress by scavenging free radicals, inhibiting lipid peroxidation, and by other mechanisms. Natural antioxidant defense systems protect biomolecules against ROS/RNS induced damage, and re-establish or maintain redox homeostasis. Seaweeds are known to produce a variety of compounds and several of them have been shown to possess biological activity of potential medicinal values. For centuries, several seaweeds have been utilized traditionally as food supplements for various medical conditions. High-performance liquid chromatographic analysis was performed for ethyl acetate fraction (E4) of S. wightii using mobile phase methanol: water ratio of 85:15flow rate 1ml/min wavelength at 254nm against reference standard phloroglucinol. For the E4 fraction a peak obtained at retention time 2.704 min and for standard phloroglucinol at 2.762 min. tailing factor of the sample is 1.430 whereas phloroglucinol 1.078. The major causes for oxidative damage include Reactive Oxygen Species (ROS). ROS are produced by all aerobic organisms and can easily react with most biological molecules including protein, lipids, lipoproteins, DNA, RNA and enzymes leading to cell or tissue injury. Experimental tumors have great importance in modeling, and HeLa cell line is one of the old and commonest cervical cancer cell lines. In the present study HeLacell lines were used to screen the anti-cancer or cytotoxicitypotential of the extracts of Sargassum wightii, using preliminary screening technique. The cytotoxicity increased with increase in concentration of phlorotannin rich extract.
Keywords: Oxidative stress, ROS/RNS, HeLacell, JNC 8 guidelines, cytotoxicity.