Friday , 21 June 2024

Method Development and Validation for the Quantitative Estimation of Citalopram in Bulk and Pharmaceutical Tablets by RP-HPLC

Bushra Nazeer and P. Bharath Rathna Kumar*
Department of Pharmaceutical Analysis & Quality Assurance, Anwarul Uloom College of Pharmacy, New Mallepally, Hyderabad, Telangana, India.

In this study, a simple, precise and accurate reverse phase high performance liquid chromatography was developed for the estimation of citalopram in bulk and pharmaceutical dosage forms. A reverse phase Merck C18column (250cm x 4.6nm x 5µ ) with mobile phase consisting of potassium dihydrogen orthophosphate and acetonitrile (70:30 v/v) ( pH 5.0 adjusted with orthophosphoric acid) was used . The flow rate was 1.0ml/min and the effluents were monitored at 224 nm. The retention time was found to be 4.69 min. The linearity of the drug was obtained in the range of 0-16ppm. The proposed method was statistically validated and applied successfully for the estimation of citalopram in API and tablet dosage form. The validation studies exposed that the developed method is rapid, accurate and reproducible. The high recovery and low relative standard deviation confirms that the suitability of the method for the routine determination of citalopram in bulk and pharmaceutical tablet dosage forms. The results of analysis have been validated according to ICH guidelines.
Keywords: Citalopram, RP-HPLC, Method Development, Validation.

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