Shaik Waseem Hussain1*, Y. Prapurna Chandra2, Venugopalaiah. Penabaka3
1PG Research Scholar, Department of Pharmacology, Ratnam Institute of Pharmacy, Nellore, A.P.
2Professor & HOD, Department of Pharmacology, Ratnam Institute of Pharmacy, Nellore, A.P.
3Asso.Professor, Department of Pharmaceutics, Ratnam Institute of Pharmacy, Nellore, A.P.
A B S T R A C T
Introduction: Cancer can be defined as the abnormal, excessive, uncontrolled proliferation of cells under the influence of a plethora of known and unknown carcinogenic stimuli. Aim: To evaluate cytotoxic potential of methanolic extract of tender prop roots obtained from Ficus benghalensis through in–vitro assays like Tryphan blue method and MTT assay emploting Colo 320 cell lines. Materials: The tender prop roots were collected from Ficus benghalensis tree was collected from the same was authenticated by the Botanist at SV Govt. arts and science college, Tirupati and the specimen was preserved for reference. The Aerial prop roots of Ficus benghalensis were shade dried and pulverized to get coarse powder followed by sieving (Sieve Number 44) to remove the unwanted waste material and to obtain the coarse powder. Results: Preclinical safety testing of recent drug candidates may be a crucial step in pharmaceutical drug development and depends on anordered series of in-vitro, in-vivo and in-silico tests before administration to humans. Conclusion: The study concludes that tissue culture technology has virtually revolutionized cancer biology in discovering cytotoxic molecules. Our current study is a preliminary effort to evaluate cytotoxic potential of methanolic prop root extract of Ficus benghalensis against colorectal adenocarcinoma cell lines Colo 320 employing two widely known in-vitro assays viz Tryphan blue and MTT assay.
Keywords: Cytotoxic potential, Colo 320, Ficus benghalensis, in-silico