Monday , 21 September 2020

Development and Validation of RP-HPLC Method for the Estimation of Olmesartan Medoxomil and Cilnidipine in Tablet Dossage Form

Devalam Madhu*, V. Hari Baskar, M. Gobinath, Ramesh Dhani, V. Pavan Kumar, N. Vamsi Krishna
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Muthukur, SPSR Nellore, A.P, India

A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Olmesartan medoxomil and Cilnidipine in Tablet dosage form. Chromatogram was run through kromosil (150mm 4.6mm, 5µ). Mobile phase containing Buffer and Acetonitrie in the ratio of 40:60 was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength for Olmesartan medoxomil and Cilnidipine was 294nm. Retention time of Olmesartan medoxomil and Cilnidipine were found to be 2.55 min and 3.92 min. %RSD of the Olmesartan medoxomil and Cilnidipine were and found to be 1.02 and 1.30respectively. %Recover was Obtained as 100.15% and 100.04% for Olmesartan medoxomil and Cilnidipine. LOD, LOQ values were obtained from regression equations of Olmesartan medoxomil and Cilnidipine were 0.14ppm, 0.10ppm and 0.41ppm, 0.30ppm respectively. Regression equation of Olmesartan medoxomil is y = 1843x + 261.1, and of Cilnidipine is y = 3611x + 213.0.
Keywords: Olmesartan medoxomil, Cilnidipine, RP-HPLC.

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