Uday Sasi Kiran Kantheti*1. K. Sonali Chowdary2, D. Rajesh Kumar3, S. Kumar Tataji4
1Dept. of Pharmacology, Royal college of Pharmacy and Health Sciences, Berhampur, Odisha, India-760002
2Department of Pharmacology, Aston University, Birmingham, UK
3Department of Pharmaceutics, Sarada College of Pharmaceutical Sciences, Narasaraopet, India
4Department of Pharmacology, Vinayaka Missions College of Pharmacy, Salem, Tamil Nadu, India
Juemingzi is the seed of the Cassia tora L.plants and has been used as a laxative, tonic, anti-aging, anticancer and anti-oxidant effects as well as a being a popular health drink. The anticancer activity of the seed was tested against the sarcoma 180 tumer bearing mice. The average tumor weight of the sarcoma 180 cell bearing mice receiving a normal diet (control) was 4.2+- 0.3 g, where as that of the mice that were treated with 50 and 100 mg/kg body weight. Juemingzi was 3.9+- 0.4 and 3.1 +- 0.4g. The extract (50, 100 and 200mg/kg b.w) treated mice demonstrated tumor growth inhibitory rates of 14.2, 31.0, and 39% respectively. The AST levels in the normal mice were 33.6+- 1.2U/ml however, that of the sarcoma 180 control mice was significantly increased to 46.9 +- 1.1 karmen U/ml. The levels of AST in the mice that were treated with 50, 100 and 200 mg/kg b.w. Juemingzi were 42.0 +- 1.1, 40.0 +- 0.6 and 37.3 +- 1.3 karmen U/ml. respectively. The ALT levels in the normal group were 22.3 +- 0.7 karmen U/ml, where as those in the control group were 36.3 +- 1.0 Karmen U/ml, reflecting marked increase. The ALT levels in the 50, 100 and 200mg/kg b.w. Juemingzi group decreased to 30.1 +- 2.1, 28.2 +- 1.4 and 25.6 +- 1.2 karmen U/ml respectively. The serum TNF alpha and IL- I beta levels of the mice in the Juemingzi treated group were significantly higher than those in control group. The increase in TNF alpha and IL-I beta levels in the 200mg/kg b.w. Juemingzi treated mice were 50.6 +- 1.8 and 470.2 +- 10.2 mg/ml, respectively compared with those of the control group. The TNF levels in the mice that were treated with 50 and 100 mg/kg b.w. Juemingzi were 36.2+- 0.6 and 40.1 +- 0.2, respectively and Il-I beta levels were 345.6 +- 18.1 and 408.2 +- 23.0 respectively. The present study provided further data indicating the in vivo anticancer effects of Juemingzi.
Key words: Cassia tora Leguminosae, Balb sarcoma 180, Mice, Antitumor activity