Analytical Method Development and Validation for the Simultaneous Estimation of Valproate and Lomitrigine by RP-HPLC Method

Kamana Nagaraju^1*, Gope Edward Raju², Kandregula Uma Maheswari³, Doonaboyina Raghava⁴, Kavala Nageswara Rao⁵
Department of Pharmaceutical Analysis, K.G.R.L College of Pharmacy, Bhimavaram- 534201, A.P, India

A B S T R A C T
A simple precise and accurate reverse phase high performance liquid chromatographic technique was developed and validated for the simultaneous estimation of Lamotrigine and Valproate in a combined dosage form Symmetry Agilent C18 (4.6*150mm) 5µm column in isocratic mode was used with the mobile phase comprising of Water and Methanol in the ratio of 40:60v/v, the flow rate was set at 1ml/min. The analyte was monitored with dual wavelength UV detector at 255nm. The retention time of Lamotrigine and Valproate was found to be 2.551 and 4.879 min respectively. The linearity range was found to lie from 10µg/ml to 50µg/ml of Lamotrigine, 20µg/ml to 100µg/ml of Valproate. Percentage recoveries were obtained in the range of for Lamotrigine 98.8% and for Valproate 98.5%. The proposed method is precise, accurate, selective, reproducible and rapid for the simultaneous estimation of Lamotrigine and Valproate in combined form.
Keywords: Lamotrigine, Valproate, UV, HPLC