Saturday , 2 March 2024

Analytical Method Development and Validation for Simultaneous Estimation of Pyrimethamine and Sulphadoxine in Pharmaceutical Dosage form by RP-HPLC

N. Kalpana Devi*1, Tirupathi Rao Velamala2, D.S.S.N.Neelima3
1,2 Teegala Krishna Reddy College of Pharmacy, Hyderabad, Telangana State

3Vikas Institite of Pharmaceutical Sciences, Rajahmundary

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Sulphadoxine and Pyrimethamine, in its pure form as well as in tablet dosage form. Chromatography was carried out on an XBridge C18 (4.6×250mm) 5µ column using a mixture of Phosphate Buffer: Acetonitrile (70:30) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 224nm. The retention time of the Pyrimethamine and Sulphadoxine was 2.8, 3.4±0.02min respectively. The method produce linear responses in the concentration range of 5-25µg/ml of Pyrimethamine and 100-750µg/ml of Sulphadoxine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Keywords: Pyrimethamine, Sulphadoxine, RP-HPLC, validation.

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