B. Saidulu*, G. Usha Sree, Dr. V. Uma Maheswara Rao
Department of Pharmaceutical Analysis and Quality Assurance, CMR College of Pharmacy, Medchal, Hyderabad, Rangareddy, India
A B S T R A C T
A reverse phased liquid chromatography (LC) method was developed and validated for simultaneous estimation of albendazole and ivermectin in tablet dosage form. The isocratic LC analysis was performed on INERTSIL C18 BDS column (250 x 4.6 mm, 5 µ) using mobile phase composed of acetonitrile, methanol and water in ratio of 40:60 (v/v) at a flow rate of 1 mL/min. Quantitation was performed using UV detector at 280 nm and the run time was 10 min. The retention times were found to be 3.9 min for albendazole and 2 min for ivermectin. The analytical method was validated according to ICH guidelines. The linearity was observed in the range of 133.7-293.4 with correlation coefficient, r=1.0 and 1 for albendazole and ivermectin respectively. The relative standard deviation values for repeatability and intermediate precision studies were less than 2%, and the accuracy (% recovery) was greater than 98% for both the drugs. The method was successfully applied for market sample analysis and mean percentage assay values were 99.99 and 100.01s for albendazole and ivermectin respectively. The present method is precise and accurate and can be used for the routine estimation of albendazole and ivermectin in tablet dosage forms.
Keywords: Albendazole, Ivermectin, RP-HPLC, validation