Saturday , 20 April 2024

Analytical Method Development and Validation by RP-HPLC for Simultaneous Estimation of Ketorolac and Febuxostat in Combined Tablet Dosage Form

Gampa Vijaya Kumar*1, Dr. P. Shashikala2
1Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Keesara, Rangareddy, Telangana, India
2Professor, Department of Pharmaceuticals and fine Chemicals, University College of Technology, Osmania University, Hyderabad, Telangana, India. 

A simple, Accurate, precise method was developed for the simultaneous estimation of the Ketorolac and Febuxostat in Tablet dosage form. Chromatogram was run through Inertsil C18 (4.6 x 150 mm, 5mm, Make: X Terra) or equivalent. Mobile phase containing methanol and phosphate buffer in the ratio of 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used at pH 3.5. Temperature was maintained at 30°C. Optimized wavelength for Ketorolac and Febuxostat was 292 nm. Retention time of Ketorolac and Febuxostat were found to be 4.981 min and 3.54 min. %RSD of the Ketorolac and Febuxostat were and found to be   less than 2% respectively. %Recovery was found to be  97% and 103% for Ketorolac and Febuxostat respectively. LOD, LOQ values are obtained from regression equations of Ketorolac and Febuxostat was found to be within the limits respectively. The linearity range of Ketorolac and Febuxostat were found to be from 10-50 mg/ml of Ketorolac and 5-25mg/ml of Febuxostat. Linear regression coefficient was not more than 0.999.
Keywords: Ketorolac, Febuxostat, RP-HPLC

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