Saturday , 4 December 2021

A New RP-HPLC method developed and validated for the estimation of Tolterodine

Gobalakrishnan P*1, Munisekhar V, Hemanth Kumar V
1Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy & Research Centre, Nellore, Andhra Pradesh, India.

A B S T R A C T
A rapid and sensitive RP-HPLC Method with UV detection (283nm) for routine analysis of Tolterodine in Bulk and in Pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of Acetonitrile and 10mm Ammonium Acetate (80:20 v/v) with flow rate 1.0 ml/min. In the range of 20-100 µg/ml, the linearity of Tolterodine shows a correlation co-efficient of 0.999. The proposed method was validated by determining sensitivity, accuracy, precision, robustness, stability, specificity and selectivity.
Keywords: Tolterodine, UV/Vis Spectrophotometer, K3Fe (CN)6, FC reagent, 2,2′ bipyridine, HPLC.

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