Asish Bhaumik1, Sanjoy Das*2, Shibu Das3, Soma Acharjee3, Prasenjit Das4
1Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda, Telangana, Pin-508206, India
2Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, Pin-786004, India
3Department of Pharmaceutical Chemistry, Regional Institute of Pharmaceutical Science and Technology, Agartala, Tripura, Pin-799005, India.
4Department of Pharmacy, Tripura University, Suryamaninagar, Tripura (W), Agartala, Tripura, Pin- 799022, India.
A B S T R A C T
The objective of the present work is to evaluate in vitro anti-oxidant and anti-arthritic activity of fruits (with seeds) of Lagerstroemia speciosa L. Based on this, a new series of constituents had been planned to extract by ethanol from the fruit-seeds of Lagerstroemia speciosa L. The in-vitro anti oxidant activity was carried out by DPPH assay and the in-vitro anti arthritic activity was performed by BSA method. The results of DPPH free radical scavenging activity showed that the ethanolic extracts of fruit-seeds of Lagerstroemia speciosa (EE-FSLS) possessed good to moderate anti-oxidant activity. The percentage (%) inhibition at various concentration (10-250 µg/ml) of EE-FSLS was found to be 14.89%, 20.53%, 31.81%, 37.62%, 49.24% and 60.52% and the IC50 value was found to be 152.78 μg/ml and the IC50 value of Std. drug ascorbic acid was found to be 97.52 μg/ml. The in-vitro experimental data of BSA method displayed that EE-FSLS inhibit the protein denaturation and the percentage of protein denaturation by EE-FSLS was found to be 29.4%; 42.5% and 59.5% etc and executed good anti-arthritic activity.
Keywords: Bioactive compounds, Anti-oxidant, Anti-arthritic activity, DPPH, BSA, IC50 etc.