Phytochemical analysis of the leaves of Tabernaemontana Corymbosa using HPLC

Naira Nayeem¹, M Someshekhar*², Saifulla Khan^3 
1Department of Pharmaceutical Chemistry, College of Pharmacy, Northern Border University, Saudi Arabia.
²Department of Pharmaceutical Chemistry. Krupanidhi College of Pharmacy, Bangalore, India
³Department of Pharmacognosy. Krupanidhi College of Pharmacy, Bangalore, India

Abstract
The study was aimed at quantifying a few phenolic compounds and sterols present in the leaves of Tabernaemontana corymbosa using HPLC. The HPLC estimation of the phytoconstituents was performed using Shimadzu High Performance Liquid Chromatographic system. The flow rate was maintained at 1 ml/min and the detection   was at 254 nm. The results of the  HPLC analysis of the leaf has shown peaks at retention times (metes) of 2.86 for Gallic acid, 3.49 for rutin, 4.13 for umbelliferone,7.16 for stigmasterol and 4.48 for sitosterol which were similar to that of the standards. The present study establishes the presence of phenolic compounds and sterols which are important plant constituents and could be responsible for the various activities exhibited and reported for the plant.
Keywords: Tabernaemontana corymbosa, HPLC, Phenolic acids, Sterols