Monday , 24 June 2024

Method Development Validation and Degradation Studies of Metoprolol and Atorvastatin by using HPLC

Sanju Srinivas1, T. Usha Rani2
1Associate Professor Department of Pharmacy, Ganga Pharmacy College, Nizamabad, Telangana
2Department of Pharmaceutical Analysis, Ganga Pharmacy College, Nizamabad, Telangana

The proposed HPLC method was found to be simple, specific, precise, accurate, rapid and economical for simultaneous estimation of and Atorvastatin in tablet dosage form. From literature review and solubility analysis initial chromatographic conditions Mobile phase ortho phosphoric acid buffer: Methanol 65:35 were set (Buffer PH 2.45 adjusted with Triethylamine), Kromosil C 18 (250×4.6mm, 5µ ) Column, Flow rate 1.0ml/min and temperature was ambient, eluent was scanned with PDA detector in system and it showed maximum absorbance at 254nm. As the methanol content was increased Metprolol and Atorvastatin got eluted with good peak symmetric properties. The retention times for Metprolol and Atorvastatin was found to be 2.589 min and 3.711 min respectively. System suitability parameters were studied by injecting the standard five times and results were well under the acceptance criteria. Linearity study was carried out between 50% to150 % levels, R2 value was found to be as 0.999.By using above method assay of marketed formulation was carried out, 100.7% was present. 

Keywords: HPLC, Atorvastatin, Metprolol, Ortho Phosphoric, Methanol, Kromosil C18.

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