Wednesday , 19 June 2024

Method Development and Validation for Simultaneous Estimation o f Bicalutamide and Atenolol by using RP-HP LC in Bulk and Pharmaceutical Dosage form

Mulla Rafiyuddin*, Akepogu Jayaraju, J. Sreeramulu
Department of Chemistry, Sri Krishnadevaraya University, Anantapuramu–515003, Andhra Pradesh, India

A B S T R A C T
A reverse phase high performance liquid chromatographic method was developed for the determination of Bicalutamide and Atenolol in bulk and pharmaceutical dosage form. The separation was carried out on a [Column: Agilent C18 (4.6 x 250mm, 5µ m, Make: Waters)] using a mobile phase mixture of water, acetonitrile in an isocratic elution at a flow rate of 1ml/min. The detection was made at 254 nm. The retention time of Bicalutamide and Atenolol was found to be 2.325 and 4.322 min respectively, Calibration curve was linear over the concentration range of 1μg – 5 μg and 100μg-500μg of Bicalutamide and Atenolol. The propose method was validated as per the ICH guidelines. The meth od was accurate, precise, specific and rapid found to be suitable for t he quantitative estimation of related substances in drug an d pharmaceutical dosage form.
Keywords: Bicalutamide and Atenolol, HPL C, Validation studies

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