Monday , 21 September 2020

Analytical Method Development and Validation by RP-HPLC for Simultaneous Estimation of Losartan Potssium and Perindopril Erbumine in Combined Tablet Dosage Form

Shaik Mubeena Yasmeen2, P. Sowjanya3, Dr. T. Rajesh4, Dr. Gampa Vijaya Kumar*1
1Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy.
2,3,4Deapartment of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy.

A B S T R A C T
A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Losartan Potassium and Perindopril erbumine in pharmaceutical dosage form. The mobile phase consisted of ortho phosphoric acid: HPLC water: 55:45 0.1ml /10 min and wavelength of detection at 218 nm. The retention times of were Losartan Potassium and Perindopril erbumine 3.016 min and 3.43 min respectively. The method was validated in terms of linearity, precision, accuracy, limit of detection, limit of quantification. The method was found to be linear in the range of inertsil-ODS C18 (250 x 4.6mm, 5μ) g/ml for Losartan potassium and Perindopril erbumine respectively. The coefficient of variance for both the drug was more than 0.999.The proposed method can be used for determination of these drugs in combined dosage forms.
Keywords: Losartan potassium, perindopril erbumine, RP-HPLC 

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