Sunday , 25 February 2024

Method development and validation by RP-HPLC for the estimation of Zolmitriptan in bulk and its dosage form

About author
D. Samson Israel*, D.Gowri Sankar,  K.Krishna Chaitanya, K.Balaram Kumar, K.Vinod
University college of Pharmaceutical sciences, Andhra University, Visakhapatnam, A.P
E-mail: [email protected]

Abstract
Zolmitriptan is used in the acute treatment of migraine attacks. It was quantified by reverse phase HPLC equipped with Sunniest (C18, 150 x 4.6 mm, 5.0 µ) column and mobile phase using pH 8.5 buffer and methanol as organic modifiers and flow rate is adjusted to 1.0 mL/min Column temperature is maintained at 40˚C. Injection volume is 10µL. the retention time was found to be 5.7 min. The standard plots were constructed between concentrations vs. peak area a linear response of peak area was observed over the concentration range of 125-750 µg/mL for Zolmitriptan The regression equations of concentration Zolmitriptan was found to be y = 324.47x + 3.400, where y is the peak area and x is the concentrations of drugs (μg/mL).
Key words: Zolmitriptan, RP-HPLC, Estimation and validation

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