Saturday , 8 May 2021

Isolation, Screening and Identification of PCP-Degrading Bacterial Strains from Sludge

Kumar Sunil1, Tandan Neeraj2 and Bharadwaj Uma1
1Department of Microbiology, Maharaj Vinayak Global University, Global University in Rajasthan, Jaipur, University, India.
2Scientific and Applied Research Centre, Meerut, India. 

A B S T R A C T
A significant number of organic compounds present in pulp-paper mill effluent have been classified as mutagenic and clastogenic thereby turning these effluents into ‘a Pandora’s box of waste chemicals’. Over last decades continuous discharge of the organic pollutants from pulp paper mill into the environment has increasing the levels of these pollutants in water system. In this thesis we assessed, physico-chemical analysis of collected effluent. The collected effluent was found slightly alkaline (pH-8.2) in nature and dark brown in colour with high level of chlorophenols PCP (220 mg/l); dichlorophenol (18.65) and trichlorophenol (10.73). Other pollution parameters like COD (15766±260 mg/l), BOD (6033±155 mg/l), color 18534 CU, nitrates (35.3±3.0 mg/l) and sulphate (405±10.2 mg/l) were also found in high level. The low BOD/COD ratio (approx 0.34) and high color of effluent are due to presence of high molecular weight compounds, i.e. PCP and lignin is complex compounds, which contribute high COD instead of BOD. Moreover, this study tended to evaluate the removal of PCP by bacterial system. In this study we were isolate 31 bacteria through enrichment culture technique. Three strains PPS-S6, S7 and S8were screened on the basis of various enzymatic activity (peroxidises and laccases enzymes) and identified as Pseudochrobactrum sp, Providencia rettgeri and Klebsiella pneumoniae, respectively on the basis of their biochemical properties.
Keywords: PCP, HPLC and GC-MS, COD.

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