J. Jayabarath*, **K. Jeyaprakesh, J. Jeny Joseline, M.J. Benuva
Head*, Department of Biotechnology,Pavendar Bharathidasan College of Engineering and Technology, Mathur, Trichy-24, India
**Head Dept of Biochemistry, Sir Rajah’s Sarafoji College of Arts & Science, Thanjavour
NCADDD, 25 July 2014, Organized by Department of Pharmaceutical Technology, Anna University, BIT Campus, Tiruchirappalli–620024, Tamil Nadu, India
Glycerol finds a lot of industrial applications in the fields of cosmetics pharmaceuticals and in industries. In the present study the production of glycerol is studied using candida krusei in a batch reactor. The influence of different carbon source on glycerol production is studied using glucose, sucrose and different combinations of glucose and sucrose mixtures. The effect of initial pH was studied by varying the initial pH from 5.0 to 9.0 in order to find the optimum pH to maximize the concentration of cellmass and glycerol. Initial pH 7.0 was found to be an optimum for glycerol fermentation. The maximum glycerol concentration (0.58g/l) was obtained when glucose is used as a carbon source at a concentration of 200g/l.
Keywords: Glycerol, candida krusei, glycerol fermentation