Naveen*, P. Naresh Babu
Department of Pharmacology, Ratnam Institute of Pharmacy, Pidathapolur, SPSR Nellore, A.P, India.
A B S T R A C T
The present study was undertaken to evaluate the antidiabetic and antioxidant effect of extract, namely ethaonol of whole plant by in vitro assays. Psychotria octosulcota whole plant were dried and subjected to ethanolic extraction. Antidiabetic activity was measured by α- glucosidase inhibitor and α- amylase inhibitor assay. Antioxidant activity was studied by measuring the total antioxidant activity by 1-1-Diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH) free radical scavenging assay, Superoxide anion radical scarvenind assay and Nitric oxide radical inhibition assay method. IC50 values of both extracts were calculated for all the assays. The results showed that ethanolic extract of whole plant of Psychotria octosulcota have the potential to inhibit alpha glucosidase with IC50 value of 0.2 5mg/ml. Similar to antidiabetic effect. Therefore,it is suggested that ethonolic extract of Psychotria octosulcota (EEPO) is a potential source for natural antidiabetic and antioxidant compounds and could have potential use in the management of diabetesmellitus.
Keywords: Psychotria octosulcota, α-glucosidase, α-amylase, antidiabetic, antioxidant