Monday , 26 February 2024

Development of new simultaneous RP-HPLC method for the estimation of Allopurinol and Lesinurad in tablet dosage form

G Surekha*, B.V. Ramana, G. Nagarajan
Dr.K.V.Subba Reddy Institute of Pharmacy, Kunrool, Andhra Pradesh, India

A simple precise, accurate and robust reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for simultaneous estimation of Allopurinol and Lesinurad. Chromatographic separation was achieved Inertsil ODS C18 column (4.6 x 150mm, 5µ) using the mobile phase consisting of Phospahte buffer 3.5 pH and Acetonitrile ratio of 70:30. The mobile phase was pumped at a flow rate of 1.5 mL/min and detection was done by UV detector at 255 nm. The retention times were 4.974 min for Allopurinol and 6.006 min for Lesinurad. The linearity ranges for Allopurinol and Lesinurad were 75-375 µg/ml and 50-250 µg/ml respectively with correlation coefficient 0.999. The proposed method was stastically validated according to ICH guidelines. All the validation parameters are present in within the limit. The developed could be applied for routine quality control analysis for simultaneous estimation of Allopurinol and Lesinurad in pharmaceutical dosage forms.
Keywords: Allopurinol, Lesinurad, RP-HPLC, Mobile phase, Retention time

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