Sunday , 3 March 2024

Development and Validation of UV-VIS Spectrophotometer Method for Estimation of Flavonoid in Tagetes Erecta

V. Amaravathi*, M. Murali, Sk. Nagoor Shareef, Thatiparthi Harshitha, Thummala Likhitha Priya
Jagans College of Pharmacy, SPSR Nellore, Andhra Pradesh, India

A b s t r a c t
For the determination of Quercetin in the Tagetes Erecta extract, a rapid, clear, selective and accurate UV-Visible Spectrophotometric method has been developed. The detection was carried out using ethyl acetate as a solvent at an absorption maximum of 369 nm. As per ICH guidelines, the procedure was validated. Quercetin was found to be 0.82±0.020w/w present in the Tagetes Erecta extract. The techniques obey the law of Beer’s-Lamberts in concentration ranges used for evaluation. The outcome of the study was statistically confirmed. The approach proposed can therefore be used to accurately measure the active marker compound in the crude drug.

Keywords: Tagetes Erecta, Beer’s-Lamberts Law, ICH guidelines, UV-visible spectrophotometry

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