Saturday , 8 May 2021

Development and Validation of Tramadol Hydrochloride Capsules by RP-HPLC Method

M. Murugeshwari*, T. Senthil kumar, N. Venkateshan
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anandnagar, Krishnankoil – 626 190, Tami Nadu.

A B S T R A C T
A simple, fast and precise reverse phase high performance liquid chromatography method was developed for the determination of Tramadol Hydrochloride in capsules by using HiQ W C18(250mm×4.6mm, i.d., 5µ) column with mobile phase of acetonitrile: methanol : 25Mm sodium dihydrogen ortho phosphate ( 30:30:40) adjusted to pH3 with 1Mm SLS was used. The flow rate was 1mL/minute and UV detection at 215nm.The retention time for Tramadol Hydrochloride was 4.7minutes.The linearity range was found to be 5.13 – 15.39µg/mL. The proposed method was validated as per ICH guidelines.

Keywords: Tramadol Hydrochloride, RP-HPLC, capsule

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